Selective Reaction Monitoring (SRM) is a mass spectroscopic analysis method utilising triple quadrupole instruments. It allows for quantitatively and highly specific detection of pre-selected analytes in a complex mixture.
Major features:
- Automated antibody-free detection method
- High analyte specificity, superior to immunoassays
- Absolute and relative quantification
- High reproducibility
- High dynamic range
- Flexible multiplexing, no cross-reactivity (up to 50-80 proteins per analysis)
- Medium throughput (200 samples/week)
- Small sample size <1ul of serum or plasma
- Cost and sample effective