Selective Reaction Monitoring

Selective Reaction Monitoring (SRM) is a mass spectroscopic analysis method utilising triple quadrupole instruments. It allows for quantitatively and highly specific detection of pre-selected analytes in a complex mixture.

Nature Methods

Major features:

  1. Automated antibody-free detection method
  2. High analyte specificity, superior to immunoassays
  3. Absolute and relative quantification
  4. High reproducibility
  5. High dynamic range
  6. Flexible multiplexing, no cross-reactivity (up to 50-80 proteins per analysis)
  7. Medium throughput (200 samples/week)
  8. Small sample size <1ul of serum or plasma
  9. Cost and sample effective